Date of Award
6-1973
Document Type
Open Access
Degree Name
Bachelor of Science
Department
Chemistry
Language
English
Abstract
The study was iniated to investigate the properties of fluorescein as a reporter group or spectral probe of protein environment. Through spectral and flourescence studies it was determined that the flourescein binding sites were non-polar relative to bulk water. The spectral study also showed a lowering of pKA for the first one or two flourescein molecules to bind to the protein.
Recommended Citation
Martin, Michael Andrew, "The Investigation of the Use of Fluorescein as a Spectral Probe of Protein Environment" (1973). Honors Theses. 1972.
https://digitalworks.union.edu/theses/1972
Rights Statement
