Date of Award

6-1999

Document Type

Union College Only

Degree Name

Bachelor of Science

Department

Chemistry

Language

English

Abstract

The regulation of actin stress fibers (SF) are linked to the stability of microtubule (M1) structures. The exact method of interaction is not understood. To study the interaction, live cells containing fluorescent α-actinin were observed and treated with MT perturbing drugs as well as ATP-depletion. These treatments affect the stability of the microtubules and stress fibers respectively. A plasmid coding for fluorescent tubulin was also inserted into these cells to aid in observing both the MTs and SFs simultaneously. The observation relies on fluorescent microscopy and digital imaging. By observing the changes in the MTs and SFs in live cells, it is hoped that the mechanism of their interaction will be shown.

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