Date of Award

6-1973

Document Type

Union College Only

Degree Name

Bachelor of Science

Department

Chemistry

Language

English

Abstract

The study was iniated to investigate the properties of fluorescein as a reporter group or spectral probe of protein environment. Through spectral and flourescence studies it was determined that the flourescein binding sites were non-polar relative to bulk water. The spectral study also showed a lowering of pKA for the first one or two flourescein molecules to bind to the protein.

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