Date of Award
Union College Only
Bachelor of Science
The study was iniated to investigate the properties of fluorescein as a reporter group or spectral probe of protein environment. Through spectral and flourescence studies it was determined that the flourescein binding sites were non-polar relative to bulk water. The spectral study also showed a lowering of pKA for the first one or two flourescein molecules to bind to the protein.
Martin, Michael Andrew, "The Investigation of the Use of Flourescein as a Spectral Probe of Protein Environment" (1973). Honors Theses. 1972.