Date of Award
Union College Only
Bachelor of Science
A recently developed method for the quantitative detection of carbonyl compounds in ambient air was investigated, specifically in order to further standardize and improve the process. The method involves drawing a gas phase sample of carbonyl compounds through impingers containing a 2, 4-Dinitrophenylhydrazine solution is then injected directly into the hplc for separation on a reversed phase column and quantitative determination by UV absorption at 254 nm and 360 nm simultaneously. The critical feature of the method (based upon a standard analytical derivatization reaction) is the use of the acetonitrile solvent. This allows for direct injection of the sampling solution into the hplc with no workup (i.e. hydrazone extraction) necessary. This modification accounts for improved detection efficiency over previous 2, 4-DNPH methods.
Kelly, Clyde A., "Determination of aldehydes and ketones in the gas phase" (1983). Honors Theses. 1891.