Date of Award
Bachelor of Science
A rapid, accurate method of determining the exact phase of a menstrual cycle at a given time is extremely important. This information is vital to doctors studying menstrual abnormalities and more importantly as an aid in conceptive and contraceptive decisions. Many methods based on changes in the body have been used to detect ovulation. These nonquantitative methods depend on the cyclical variations in basal boy temperature, physical and chemical properties of mucus and content of saliva. These changes are influenced by changing hormone levels. Specicially, estrogen levels rise several days prior to ovulation, therefore, methods for determining estrogen concentration in blood and urine are of interest. Most methods involve hydrolysis and purification followed by radioimmunoassay, colorimetry, fluorimetry or enzymatic determinations. None of the methods proved satisfactory due to accuracy limitations or time demands.
Lanni, Maria, "A Survey of Detection Methods for Ovulation and a Proposed Method for Estrogen Determination" (1981). Honors Theses. 1882.